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Nov 15, 2019
Zemb, Olivier, 2019, "Absolute quantitation of microbes using 16S rRNA gene metabarcoding: a rapid normalization of relative abundances by quantitative PCR targeting a 16S rRNA gene spike-in standard", https://doi.org/10.15454/YWMMH4, Portail Data INRAE, V1, UNF:6:F0zGeneCWQ7Kv1ouvU+bEQ== [fileUNF]
Metabarcoding of the 16S rRNA gene is commonly used to characterize microbial communities, by estimating the relative abundance of microbes. Here we present a method to retrieve the concentrations of the 16S rRNA gene per gram of any environmental sample using a synthetic standar...
Jul 30, 2019
Pauvert, Charlie; Vallance, Jessica; Delière, Laurent; Buée, Marc; Vacher, Corinne, 2019, "Amplicon Sequence Variant (ASV) table obtained by metabarcoding foliar fungal communities in conventional and organic vineyards", https://doi.org/10.15454/WOICSE, Portail Data INRAE, V1, UNF:6:PSwFBpb4Vxgr5rwUlkcPUQ== [fileUNF]
This Amplicon Sequence Variant (ASV) table was obtained by metabarcoding foliar fungal communities in conventional and organic vineyards. This table was generated after the application of the bioinformatics scripts (at https://doi.org/10.15454/NSHUAQ) to the sequences available a...
Jul 12, 2019 - Grapevine Downy Mildew Genomics Dataverse
Dussert, Yann; Gouzy, Jerome; Richart-Cervera, Sylvie; Mazet, Isabelle; Deliere, Laurent; Couture, Carole; Legrand, Ludovic; Piron, Marie-Christine; Bouchez, Olivier; Mestre, Pere; Delmotte, Francois, 2019, "Gene annotation of the Illumina draft of the Plasmopara viticola genome", https://doi.org/10.15454/NQEX46, Portail Data INRAE, V1
This dataset includes the gene annotation for the Illumina draft of the Plasmopara viticola genome. This genome draft has been published in Dussert et al. 2016 (doi.org/10.1128/genomeA.00987-16). The assembly can be found on GenBank (accession GCA_001695595.1).
May 9, 2019
Pauvert, Charlie; Vallance, Jessica; Delière, Laurent; Buée, Marc; Vacher, Corinne, 2019, "Bioinformatic scripts to assess the effect of the cropping system on both community and network α- and β-properties of fungal microbiota", https://doi.org/10.15454/NSHUAQ, Portail Data INRAE, V1
These bioinformatics scripts were applied to the sequences available at https://doi.org/10.15454/3DPFNJ. They are based on several softwares, including SparCC (Friedman and Alm 2012) and DADA2 (Callahan et al. 2016). The code corresponds to R and R markdown files. These scripts w...
May 7, 2019
Pauvert, Charlie; Vallance, Jessica; Delière, Laurent; Buée, Marc; Vacher, Corinne, 2019, "DNA metabarcoding of foliar fungal communities in conventional and organic vineyards", https://doi.org/10.15454/3DPFNJ, Portail Data INRAE, V1
This sequence dataset (FASTQ format) was obtained by metabarcoding foliar fungal communities in conventional and organic vineyards. The ribosomal internal transcribed spacer region 1 (ITS1) was amplified with the ITS1F / ITS2 primer pair and sequenced on an Illumina MiSeq platfor...
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